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Adenylate kinase 1, aa1-194, Recombinant, Human (Adenylate kinase isoenzyme 1, mitochondrial, ADK2, ATP-AMP transphosphorylase)

Cat no: A0882-65D

Adenylate kinase 1, aa1-194, Recombinant, Human (Adenylate kinase isoenzyme 1, mitochondrial, ADK2, ATP-AMP transphosphorylase)

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia.\n\nSource:\nRecombinant corresponding to aa1-194 of human recombinant AK1 protein, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE\nTVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV \nIAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0882-65D

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 10% Glycerol.

Purity

~95% (SDS-PAGE)

References

1. Terzic A., et al. (2007) J Biol Chem. 282(43):31366-72. 2. Morelli A., et al (2007) Curr Eye Res. 32(3):249-57.

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