Adipocyte Fatty Acid Binding Protein (AFABP, FABP4)

Adipocyte fatty acid binding protein AFABP is a 15kD member of the intracellular fatty acid binding protein (FABP) family, which is known for the ability to bind fatty acids and related compounds (bile acids or retinoids) in an internal cavity. AFABP is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells . In mice, targeted mutations in AFABP provide significant protection from hyperinsulinemia and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from AFABP-deficient mice also have reduced efficiency of lipolysis in vitro and in vivo, and these mice exhibited moderately improved systemic dyslipidemia. Recent studies also demonstrated AFABP expression in macrophages upon differentiation and activation. In these cells, AFABP modulates inflammatory responses and cholesterol ester accumulation, and total or macrophage-specific AFABP deficiency confers dramatic protection against atherosclerosis in the apoE-/- mice. These results indicate a \ncentral role for AFABP in the development of major components of the metabolic syndrome through its distinct actions in adipocytes and macrophages. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stabillty:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F0019-73A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O. Let the lyophilized pellet dissolve completely.

P Type

Pab

Purity

Purified by Immunoaffinity chromatography.

Isotype

IgG

References

1. Prinsen CF, de Bruijn DR, Merkx GF, Veerkamp JH. Assignment of the human adipocyte fatty \nacid-binding protein gene (FABP4) to chromosome 8q21 using somatic cell hybrid and \nfluorescence in situ hybridization techniques. \n2. van Dongen MJ, Uppenberg J, Svensson S, Lundback T, Akerud T, Wikstrom M, Schultz J. \nStructure-based screening as applied to human FABP4: a highly efficient alternative to HTS for \nhit generation. \n3. Witthuhn BA, Bernlohr DA. Upregulation of bone morphogenetic protein GDF-3/Vgr-2 \nexpression in adipose tissue of FABP4/aP2 null mice.

Additional Info

Recognizes human FABP4.