Adiponectin (HEK)

Size

100ug

Hosts

Rabbit

Form

Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O.

Purity

Purified by Immunoaffinity chromatography.

References

1. Ebinuma H, Miyazaki O, Yago H, Hara K, Yamauchi T, Kadowaki T. A novel ELISA system for \nselective measurement of human adiponectin multimers by using proteases. Clin Chim Acta. \n2006 Oct;372 (1-2):47-53 \n2. Nakano Y, Tajima S, Yoshimi A, Akiyama H, Tsushima M, Tanioka T, Negoro T, Tomita M, Tobe T. A \nnovel enzyme-linked immunosorbent assay specific for high-molecular-weight adiponectin. J \nLipid Res. 2006 Jul;47 (7):1572-82 \n3. Berg AH, Combs TP, Scherer PE. ACRP30/adiponectin: an adipokine regulating glucose and \nlipid metabolism. Trends Endocrinol Metab. 2002;13:84-89. \n4. Iwashima Y, Horio T, Kumada M, Suzuki Y, Kihara S, Rakugi H, Kawano Y, Funahashi T, Ogihara T. \nAdiponectin and renal function, and implication as a risk of cardiovascular disease. Am J \nCardiol. 2006 Dec 15;98 (12):1603-8 \n5. Ahima R, Schraw T, Kos K, McTernan PG, Kusminski CM, Scherer PE, Kumar S, O'Hare JP. \nAdiponectin complexes in human cerebrospinal fluid: distinct complex distribution from serum. \nDiabetologia. 2007 Mar;50 (3):634-42 \n6. Wang Y, Lam KS, Xu JY, Lu G, Xu LY, Cooper GJ, Xu A. Adiponectin inhibits cell proliferation by \ninteracting with several growth factors in an oligomerization-dependent manner. \nPage 2 of 7 (VERSION: 2009-10-25)\nJ Biol Chem. 2005 May 6;280 (18):18341-7 \n7. Klein RL, Garvey WT, Lara-Castro C, Wallace P, Luo N. Adiponectin multimeric complexes and \nthe metabolic syndrome trait cluster. Diabetes. 2006 Jan;55 (1):249-59 \n8. Whitehead JP, Richards AA, Prins JB, Stephens T, Charlton HK, Jones A, Macdonald GA. \nAdiponectin multimerization is dependent on conserved lysines in the collagenous domain: \nevidence for regulation of multimerization by alterations in posttranslational modifications. Mol \nEndocrinol. 2006 Jul;20 (7):1673-87 \n9. Okamoto Y, Kihara S, et al. Adiponectin reduces atherosclerosis in apolipoprotein E-deficient \nmice. Circulation. 2002;106:2767-2770. \n10. Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, \nEto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki \nT. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP- \nactivated protein kinase. Nat Med. 2002; 8:1288-1295. \n11. Chen H, Montagnani M, Funahashi T, Shimomura I, Quon MJ. Adiponectin stimulates production \nof nitric oxide in vascular endothelial cells. J Biol Chem. 2003;278:45021-45026. \n12. Grambow SC, Freemark MS, Haqq AM, Muehlbauer M, Newgard CB, Svetkey LP, Purnell JQ. \nAltered distribution of adiponectin isoforms in children with Prader-Willi syndrome (PWS): \nassociation with insulin sensitivity and circulating satiety peptide hormones. Clin Endocrinol \n(Oxf). 2007 Dec;67 (6):944-51 \n13. Sinha MK, Songer T, Xiao Q, Sloan JH, Wang J, Ji S, Alborn WE, Davis RA, Swarbrick MM, \nStanhope KL, Wolfe BM, Havel PJ, Schraw T, Konrad RJ, Scherer PE, Mistry JS. Analytical validation \nand biological evaluation of a high molecular-weight adiponectin ELISA. Clin Chem. 2007 Dec;53 \n(12):2144-51 \n14. Havel PJ, Wolfe BM, Swarbrick MM, Austrheim-Smith IT, Stanhope KL, Van Loan MD, Ali MR. \nCirculating concentrations of high-molecular-weight adiponectin are increased following Roux- \nen-Y gastric bypass surgery. Diabetologia. 2006 Nov;49 (11):2552-8 \n15. Komura N, Kihara S, Sonoda M, Kumada M, Fujita K, Hiuge A, Okada T, Nakagawa Y, Tamba S, \nKuroda Y, Hayashi N, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, Arita Y, Okamoto Y, \nShimomura I, Funahashi T, Matsuzawa Y. Clinical significance of high-molecular weight form of \nadiponectin in male patients with coronary artery disease. Circ J. 2008 Jan;72 (1):23-8 \n16. Scherer PE, Pajvani UB, Hawkins M, Rajala MW, Combs TP, Doebber T, Berger JP, Wagner JA, Wu \nM, Knopps A, Xiang AH, Utzschneider KM, Kahn SE, Olefsky JM, Buchanan TA. Complex \ndistribution, not absolute amount of adiponectin, correlates with thiazolidinedione-mediated \nimprovement in insulin sensitivity. J Biol Chem. 2004 Mar 26;279 (13):12152-62 \n17. Fukui H, Uemura M, Akahane T, Tsujimoto T, Yamazaki M, Namisaki T, Yanase K, Yoshii J, Kaji K, \nKitade M, Ikenaka Y, Noguchi R, Yoshiji H, Asada K. Crosstalk between high-molecular-weight \nadiponectin and T-cadherin during liver fibrosis development in rats. Int J Mol Med. 2007 Nov;20 \n(5):725-9 \n18. Hanley AJ, Retnakaran R, Connelly PW, Maguire G, Sermer M, Zinman B. Decreased high- \nmolecular-weight adiponectin in gestational diabetes: implications for the pathophysiology of \nType 2 diabetes. Diabet Med. 2007 Mar;24 (3):245-52 \n19. Bernstein LE, Canavan B, Torriani M, Jackson MB, Ahima RS, Grinspoon SK, Lo J. Effects of TNF- \nalpha neutralization on adipocytokines and skeletal muscle adiposity in the metabolic syndrome. \nAm J Physiol Endocrinol Metab. 2007 Jul;293 (1):E102-9 \n20. Chen TH, Chen L, Hsieh MS, Chang CP, Chou DT, Tsai SH. Evidence for a protective role for \nadiponectin in osteoarthritis. Biochim Biophys Acta. 2006 Aug;1762 (8):711-8 \n21. Oki K, Koide J, Nakanishi S, Nakashima R, Yamane K. Fenofibrate increases high molecular \nweight adiponectin in subjects with hypertriglyceridemia. Endocr J. 2007 Jun;54 (3):431-5 \n22. Andersen KK, Frystyk J, Wolthers OD, Heuck C, Flyvbjerg A. Gender differences of oligomers and \ntotal adiponectin during puberty: a cross-sectional study of 859 Danish school children. J Clin \nEndocrinol Metab. 2007 May;92 (5):1857-62 \n23. Waki H, Yamauchi T, Kamon J, Kita S, Ito Y, Hada Y, Uchida S, Tsuchida A, Takekawa S, Kadowaki \nPage 3 of 7 (VERSION: 2009-10-25)\nT. Generation of globular fragment of adiponectin by leukocyte elastase secreted by monocytic \ncell line THP-1. Endocrinology. 2005 Feb;146(2):790-6. Epub 2004 Nov 4. \n24. Kasai K, Matsuoka H, Suzuki K, Nishikimi T, Akimoto K, Hattori S, Hattori Y. Globular adiponectin \nactivates nuclear factor-kappaB and activating protein-1 and enhances angiotensin II-induced \nproliferation in cardiac fibroblasts. Diabetes. 2007 Mar;56 (3):804-8 \n25. Hughes JT, Shen Y, Charlesworth JA, Peake PW. Glycosylation of human adiponectin affects its \nconformation and stability. J Mol Endocrinol. 2007 Jul;39 (1):45-52 \n26. Odden N, Morkrid L. High molecular weight adiponectin dominates in cord blood of newborns \nbut is unaffected by pre-eclamptic pregnancies. Clin Endocrinol (Oxf). 2007 Dec;67 (6):891-6 \n27. Brenner DA, Adachi M. High molecular weight adiponectin inhibits proliferation of hepatic \nstellate cells via activation of adenosine monophosphate-activated protein kinase. Hepatology. \n2008 Feb;47 (2):677-85 \n28. Buechler C, Weigert J, Sigruener A, Eggenhofer E, Scholmerich J, Neumeier M, Schmitz G, \nLangmann T, Piso P, Schaeffler A, Aslanidis C, Schlitt HJ, Weiss TS. High molecular weight \nadiponectin reduces apolipoprotein B and E release in human hepatocytes. Biochem Biophys \nRes Commun. 2007 Jan 12;352 (2):543-8 \n29. Seino Y, Hirose H, Saito I, Itoh H. High molecular weight multimer form of adiponectin as a \nuseful marker to evaluate insulin resistance and metabolic syndrome in Japanese men. \nMetabolism. 2007 Nov;56 (11):1493-9 \n30. Araki S, Dobashi K, Kubo K, Asayama K, Shirahata A. High molecular weight, rather than total, \nadiponectin levels better reflect metabolic abnormalities associated with childhood obesity. J \nClin Endocrinol Metab. 2006 Dec;91 (12):5113-6 \n31. Dugi KA, Bierhaus A, Nawroth PP, Allolio B, Hamann A, Lepper PM, Bluemm A, Humpert PM, von \nEynatten M. High-molecular weight adiponectin is independently associated with the extent of \ncoronary artery disease in men. Atherosclerosis. 2008 Jul;199 (1):123-8 \n32. Salani B, Briatore L, Adami GF, Andraghetti G, Maggi D, Cordera R. High-molecular weight \nadiponectin isoforms increase after biliopancreatic diversion in obese subjects. Obesity (Silver \nSpring). 2006 Sep;14 (9):1511-4 \n33. Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four \nconserved lysine residues in the collagenous domain of adiponectin. Potential role in the \nmodulation of its insulin-sensitizing activity. J Biol Chem. 2002; 277:19521-19529. \n34. Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four \nconserved lysine residues in the collagenous domain of adiponectin. Potential role in the \nmodulation of its insulin-sensitizing activity. J Biol Chem. 2002; 277:19521-19529. \n35. Torigoe M, Matsui H, Ogawa Y, Murakami H, Murakami R, Cheng XW, Numaguchi Y, Murohara T, \nOkumura K. Impact of the high-molecular-weight form of adiponectin on endothelial function in \nhealthy young men. Clin Endocrinol (Oxf). 2007 Aug;67 (2):276-81 \n36. Waki H, Yamauchi T, Kamon J, Ito Y, Uchida S, Kita S, Hara K, Hada Y, Vasseur F, Froguel P, \nKimura S, Nagai R, Kadowaki T. Impaired multimerization of human adiponectin mutants \nassociated with diabetes. Molecular structure and multimer formation of adiponectin. J Biol \nChem. 2003 Oct 10;278 (41):40352-63 \n37. Schober F, Neumeier M, Weigert J, Wurm S, Wanninger J, Schaffler A, Dada A, Liebisch G, Schmitz \nG, Aslanidis C, Buechler C. Low molecular weight adiponectin negatively correlates with the waist \ncircumference and monocytic IL-6 release. Biochem Biophys Res Commun. 2007 Oct 5;361 (4):968- \n73 \n38. Hamilton JK, Connelly PW, Sermer M, Maguire G, Zinman B, Hanley AJ, Retnakaran R, Ong GK. \nMaternal serum adiponectin and infant birthweight: the role of adiponectin isoform distribution. \nClin Endocrinol (Oxf). 2007 Jul;67 (1):108-14 \n39. Hara K, Horikoshi M, Yamauchi T, Yago H, Miyazaki O, Ebinuma H, Imai Y, Nagai R, Kadowaki T. \nMeasurement of the high-molecular weight form of adiponectin in plasma is useful for the \nprediction of insulin resistance and metabolic syndrome. Diabetes Care. 2006 Jun;29 (6):1357-62 \n40. Tonelli J, Li W, Kishore P, Pajvani UB, Kwon E, Weaver C, Scherer PE, Hawkins M. Mechanisms of \nearly insulin-sensitizing effects of thiazolidinediones in type 2 diabetes. \nPage 4 of 7 (VERSION: 2009-10-25)\nDiabetes. 2004 Jun;53 (6):1621-9 \n41. Hemi R, Yissachar E, Modan-Moses D, Pariente C, Kanety H, Stein D, Yaroslavsky A, Ram A, Faigin \nM, Loewenthal R. Modulation of adiponectin and leptin during refeeding of female anorexia \nnervosa patients. J Clin Endocrinol Metab. 2007 May;92 (5):1843-7 \n42. Macdonald GA, Prins JB, Hickman IJ, Wang AY, Jones AL, Newell F, Richards AA, Mowry BJ, \nWhitehead JP. Olanzapine treatment is associated with reduced high molecular weight \nadiponectin in serum: a potential mechanism for olanzapine-induced insulin resistance in \npatients with schizophrenia. J Clin Psychopharmacol. 2006 Jun;26 (3):232-7 \n43. Semple RK, Halberg NH, Burling K, Soos MA, Schraw T, Luan J, Cochran EK, Dunger DB, Wareham \nNJ, Scherer PE, Gorden P, O'Rahilly S. Paradoxical elevation of high-molecular weight \nadiponectin in acquired extreme insulin resistance due to insulin receptor antibodies. Diabetes. \n2007 Jun;56 (6):1712-7 \n44. Bodles AM, Banga A, Rasouli N, Ono F, Kern PA, Owens RJ. Pioglitazone increases secretion of \nhigh-molecular-weight adiponectin from adipocytes. Am J Physiol Endocrinol Metab. 2006 \nNov;291 (5):E1100-5 \n45. Scherer PE, Hawkins M, Halberg N, Wang ZV, Schraw T. Plasma adiponectin complexes have \ndistinct biochemical characteristics. Endocrinology. 2008 May;149 (5):2270-82 \n46. Schraw T, Wang ZV, Halberg N, Hawkins M, Scherer PE. Plasma adiponectin complexes have \ndistinct biochemical characteristics. Endocrinology. 2008 May;149 (5):2270-82 \n47. Wang Y, Lam KS, Chan L, Chan KW, Lam JB, Lam MC, Hoo RC, Mak WW, Cooper GJ, Xu A. Post- \ntranslational modifications of the four conserved lysine residues within the collagenous domain \nof adiponectin are required for the formation of its high molecular weight oligomeric complex. J \nBiol Chem. 2006 Jun 16;281 (24):16391-400 \n48. Westphal S, Luley C. Preferential increase in high-molecular weight adiponectin after niacin. \nAtherosclerosis. 2008 May;198 (1):179-83 \n49. Tajima N, Nishimura R, Morimoto A, Matsudaira T, Miyashita Y, Sano H, Shirasawa T, Takahashi E. \nRatio of high-, medium-, and low-molecular weight serum adiponectin to the total adiponectin \nvalue in children. J Pediatr. 2007 Nov;151 (5):545-7, 547.e1-2 \n50. Matsuda M, Shimomura I, et al. Role of adiponectin in preventing vascular stenosis. The \nmissing link of adipo-vascular axis. J Biol Chem. 2002;277:37487-37491. \n51. Basu R, Pajvani UB, Rizza RA, Scherer PE. Selective downregulation of the high molecular \nweight form of adiponectin in hyperinsulinemia and in type 2 diabetes: differential regulation \nfrom nondiabetic subjects. Diabetes. 2007 Aug;56 (8):2174-7 \n52. Takemura Y, Osuga Y, Koga K, Tajima T, Hirota Y, Hirata T, Morimoto C, Harada M, Yano T, \nTaketani Y. Selective increase in high molecular weight adiponectin concentration in serum of \nwomen with preeclampsia. J Reprod Immunol. 2007 Feb;73 (1):60-5 \n53. Kobayashi H, Ouchi N, Kihara S, Walsh K, Kumada M, Abe Y, Funahashi T, Matsuzawa Y, Ferre P. \nSelective Suppression of Endothelial Cell Apoptosis by the High Molecular Weight. \n54. Kobayashi H, Ouchi N, Kihara S, Walsh K, Kumada M, Abe Y, et al. Selective suppression of \nendothelial cell apoptosis by the high molecular weight form of adiponectin. Circulation research. \n2004 Mar 5;94(4):e27-31. \n55. Kobayashi H, Ouchi N, Kihara S, Walsh K, Kumada M, Abe Y, Funahashi T, Matsuzawa Y. Selective \nsuppression of endothelial cell apoptosis by the high molecular weight form of adiponectin. \nCirc Res. 2004 Mar 5;94 (4):e27-31 \n56. Hui CK, Zhang HY, Lee NP, Chan W, Yueng YH, Leung KW, Lu L, Leung N, Lo CM, Fan ST, Luk JM, \nXu A, Lam KS, Kwong YL, Lau GK. Serum adiponectin is increased in advancing liver fibrosis and \ndeclines with reduction in fibrosis in chronic hepatitis B. J Hepatol. 2007 Aug;47 (2):191-202 \n57. Kumar S, Scherer PE, McTernan PG, Barnett AH, Hanif W, Trujillo ME, Fisher FF. Serum high \nmolecular weight complex of adiponectin correlates better with glucose tolerance than total \nserum adiponectin in Indo-Asian males. Diabetologia. 2005 Jun;48 (6):1084-7 \n58. Nakatani H, Hirose H, Yamamoto Y, Saito I, Itoh H. Significance of leptin and high-molecular \nweight adiponectin in the general population of Japanese male adolescents. Metabolism. 2008 \nFeb;57 (2):157-62 \nPage 5 of 7 (VERSION: 2009-10-25)\n59. Pajvani UB, Du X, Combs TP, Berg AH, Rajala MW, Schulthess T, Engel J, Brownlee M, Scherer PE. \nStructure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications \nfpr metabolic regulation and bioactivity. J Biol Chem. 2003; 278:9073-85. \n60. Pajvani UB, Du X, Combs TP, Berg AH, Rajala MW, Schulthess T, Engel J, Brownlee M, Scherer PE. \nStructure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications \nfpr metabolic regulation and bioactivity. J Biol Chem. 2003; 278:9073-85. \n61. Hug C, Wang J, Ahmad NS, Bogan JS, Tsao TS, Lodish HF. T-cadherin is a receptor for \nhexameric and high-molecular-weight forms of Acrp30/adiponectin. Proc Natl Acad Sci U S A. \n2004 Jul 13;101 (28):10308-13 \n62. Xu A, Chan KW, Hoo RL, Wang Y, Tan KC, Zhang J, Chen B, Lam MC, Tse C, Cooper GJ, Lam KS. \nTestosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its \nsecretion from adipocytes. J Biol Chem. 2005 May 6;280 (18):18073-80 \n63. Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte-secreted protein Acrp30 \nenhances hepatic insulin action. Nat Med. 2001; 7:947-953. \n64. Beulens JW, van Loon LJ, Kok FJ, Pelsers M, Bobbert T, Spranger J, Helander A, Hendriks HF. The \neffect of moderate alcohol consumption on adiponectin oligomers and muscle oxidative \ncapacity: a human intervention study. Diabetologia. 2007 Jul;50 (7):1388-92 \n65. Xu A, Wang Y, Keshaw H, Xu LY, Lam KS, Cooper GJ. The fat-derived hormone adiponectin \nalleviates alcoholic and nonalcoholic fatty liver diseases in mice. J Clin Invest. 2003; 112:91-100. \n66. Yamauchi T, Kamon J, et al. The fat-derived hormone adiponectin reverses insulin resistance \nassociated with both lipoatrophy and obesity. Nat Med. 2001;7:941-946. \n67. Kriketos AD, Peake PW, Charlesworth JA, Campbell LV, Shen Y. The metabolism of isoforms of \nhuman adiponectin: studies in human subjects and in experimental animals. Eur J Endocrinol. \n2005 Sep;153 (3):409-17 \n68. Tsao TS, Wert D, Wilson-Kubalek EM, Suzuki S, Lee DH. The oligomeric structure of high \nmolecular weight adiponectin. FEBS Lett. 2007 Mar 6;581 (5):809-14 \n69. Shulman GI, Rothman DL, Petersen KF, Dufour S, Savage DB, Bilz S, Solomon G, Yonemitsu S, \nCline GW, Befroy D, Zemany L, Kahn BB, Papademetris X. The role of skeletal muscle insulin \nresistance in the pathogenesis of the metabolic syndrome. Proc Natl Acad Sci U S A. 2007 Jul \n31;104 (31):12587-94 \n70. Lodish HF, Hug C. The role of the adipocyte hormone adiponectin in cardiovascular disease. \nCurr Opin Pharmacol. 2005 Apr;5 (2):129-34 \n71. Glintborg D, Frystyk J, Hojlund K, Andersen KK, Henriksen JE, Hermann AP, Hagen C, Flyvbjerg A, \nAndersen M. Total and high molecular weight (HMW) adiponectin levels and measures of glucose \nand lipid metabolism following pioglitazone treatment in a randomized placebo-controlled study \nin polycystic ovary syndrome. Clin Endocrinol (Oxf). 2008 Feb;68 (2):165-74 \n72. Horie M, Yamamoto T, Fujii M, Ishikawa C, Sakai H, Tanaka T, Tsutamoto T. Total and high \nmolecular weight adiponectin, haemodynamics, and mortality in patients with chronic heart \nfailure. Eur Heart J. 2007 Jul;28 (14):1723-30 \n73. Sweeney G, Tungtrongchitr R, Shaw C, Liu Y, Retnakaran R, Hanley A. Total and high molecular \nweight but not trimeric or hexameric forms of adiponectin correlate with markers of the \nmetabolic syndrome and liver injury in Thai subjects. J Clin Endocrinol Metab. 2007 Nov;92 \n(11):4313-8 \n74. Bluher M, Mantzoros CS, Williams CJ, Kralisch S, Fasshauer M, Kelesidis T, Kratzsch J, Brennan \nAM. Total and high-molecular weight adiponectin in relation to metabolic variables at baseline \nand in response to an exercise treatment program: comparative evaluation of three assays. \nDiabetes Care. 2007 Feb;30 (2):280-5 \n75. Peake PW, Shen YY, Charlesworth JA, Kelly JJ, Loi KW. Up-regulation of adiponectin, its \nisoforms and receptors in end-stage kidney disease. Nephrol Dial Transplant. 2007 Jan;22 (1):171- \n8