Adipophilin (ADFP, Adipose Differentiation-related Protein)

Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. New specific marker for liposarcoma.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applicatons not tested.\n\nRecommended Dilution: \nWestern Blot: 1:2000\nImmunohistochemistry (Frozen, Paraffin embedded tissue after microwave treatment): 1:100; Incubate for 1hr at RT or overnight at 4 degrees C. \nOptimal dilutions to be determined by the reseracher.\n\nIHC Positive Controls:\nGlandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertolis cells of the testis, fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver (adipocytes are negative), lipid storing CD68-positive macrophages.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0887-05

Size

100ul

Applications

ICC, IHC, WB

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Heid, H.W., et al., Cell Tissue Res. 294: 309-321 (1998). 2. Heid, H.W., et al., Biochem. J. 320: 1025-1030 (1996). 3. Mrozinzki, J., et al., Adipophilin and perilipin, two lipid droplet-associated proteins, in the pathohistological differential diagnosis of human soft tissue sarcomas. Abstract from AEK meeting 1999, German Cancer Research Center. J. Cancer Res. & Clin. Oncol. 125 suppl. (1999). 4. Steiner, S., et al., Biochem. Biophys. Res. 218: 777-782 (1996). 5. Hope, R.G. and McLauchlan, J.J., General Virology 81: 1913-1925 (2000). 6. Ohsaki, Y., et al., Histochem. Cell Biol. 124: 445-452 (2005).

Additional Info

Recognizes Adipophilin at ~52kD. Reactive with cell lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelia), RV, PC-12 (rat adrenal grand), MDCK. Does not react with glioma. Species Crossreactivity: Human, mouse, rat, canine, bovine.