Adipsin

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology. Adipsin is a serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot:1-5 ug/ml using ECL. Adipsin is ~22 kD\nELISA: 1:10,000-50,000. Coat 10-100ng of A0890-01 control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nA0890-02: Adipsin, Human (Control Protein for Western Blot)\nA0890-01: Adipsin (Blocking Peptide, ELISA Standard)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0890

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Zhu, L., et al., J. Clin. Nvest. 94: 1163 (1994). 2. Baker, B.C., et al., 279: 775 (1991). 3. Min, H.Y., et al., Nucl Acid Res. 14: 8879. 5. White, R.T., et al., JBC 267: 9210 (1992). 6. Niemann, M.A., et al., Biochem. 23: 2482 (1984). 7. Johnson, D.M., et al., Bicohem. J. 187: 863 (1980). 8. Volanakis, J.E., et al., PNAS 77: 1116 (1980).

Additional Info

Recognizes human Adipsin.