Adipsin

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology.\n\nAdipsin is a serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Adipsin, also called ADN or complement factor D or C3 convertase activator or properdin factor D (precursors: mouse 259aa; rat 263aa, human 253aa, mature protein 26-253, ~22kD) cleaves factor B when the latter is complexed with factor C3B, activating the C3BB complex, which then becomes C3 convertase of the alternative pathway. Adipsin is the major protein secreted by the adipocytes. Unlike rodents, adipsin is also expressed in monocytes/macrphages. Most adipsin is secreted in blood (50ug/ml in normal lean mice and 50-100 fold less in fat from db/db or ob/ob or MSG (monosodium glutamate-treated mice). Its expression is induced upon differentiation of preadipocytes.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot:1:1000-1:5000 using ECL. Adipsin is ~22 kD\nELISA: 1:10,000-50,000. Use 10-100ng of A0890-04 control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: A0890-04\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0890-05

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS containing 0.05% sodium azide and 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Zhu, L., et al., J. Clin. Nvest. 94: 1163 (1994). 2. Baker, B.C., et al., 279: 775 (1991). 3. Min, H.Y., et al., Nucl Acid Res. 14: 8879. 5. White, R.T., et al., JBC 267: 9210 (1992). 6. Niemann, M.A., et al., Biochem. 23: 2482 (1984). 7. Johnson, D.M., et al., Bicohem. J. 187: 863 (1980). 8. Volanakis, J.E., et al., PNAS 77: 1116 (1980).

Additional Info

Recognizes rat adipsin. Species Sequence Homology: Mouse: 65%. Recommend using human adipsin for human studies.