Adipsin (Factor D)

Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology. \nAdipsin is serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Adipsin, also called ADN or complement factor D or C3 convertase activator or properdin factor D (precursors: mouse 259-aa; rat 263 aa, human 253 aa, mature protein 26-253, ~22kD) cleaves factor B when the latter is complexed with factor C3B, activating the C3BB complex, which then becomes C3 convertase of the alternative pathway. Adipocyte is the major protein secreted by the adipocytes. Unlike rodents, adipsin is also expressed in monocytes/macrphages. Most adipsin is secreted in blood (50ug/ml in normal lean mice and 50-100 fold less in fat from db/db or ob/ob or MSG (monosodium glutamate-treated mice). Its expression is induced upon differentiation of preadipocytes.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 1-5ug/ml using ECL. Human Adipsin is ~22kD.\nELISA: 1:10,000-1:50,000\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0890B

Size

50ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a liquid in 100mM Tris, pH 7.5, 0.05% sodium azide

P Type

Pab

Purity

Purified by salt fractionation, Ion-Exchange and Protein A affinity chromatography

Isotype

IgG

References

1. White RT et al (1992) JBC 267, 9210; 2. Niemann MA et al (1984) Biochem. 23, 2482; 3. Johnson DM et al (1980) Bicohem. J. 187, 863; 4. Volanakis Je et al (1980) PNAS 77, 1116; 5. Zhu L et al (1994) J. Clin. Invest. 94, 1163; 6. Baker BC et al (1991) 279, 775; 7. Min HY et al (196) Nucl Acid Res. 14, 8879;

Additional Info

Recognizes human Adipsin. Gives a single arc in IEP and Ouchterlony against the human serum.