ADP Assay Kit, BioAssay(TM)

ADP Assay Kit provides a rapid method to measure ADP levels. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. Luciferase ATP+D-luciferin+O2 oxyluciferin+AMP+PPi+CO2+light\n\nIn the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. The second light intensity measured represents the total ADP and ATP concentration in the sample. \n\nThis non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.\n\nKey Features:\nSafe. Non-radioactive assay.\nSensitive and accurate. As low as 0.02uM ADP can be quantified.\nHomogeneous and convenient. "Mix-incubate-measure" type assay.\nNo wash and reagent transfer steps are involved.\nRobust and amenable to HTS: Z

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SPECIFICATIONS

Catalog Number

A0901-75A

Size

1Kit

References

1. Schwarzer C., et al. (2008). Oxidative stress caused by pyocyanin impairs CFTR Cl(-) transport in human bronchial epithelial cells. Free Radic. Biol. Med. 45(12): 1653-62.\n2. Chandak P.G., et al. (2010). Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase. J Biol. Chem. 285(26): 20192-201.\n3. Belleanne?e C., et al. (2010). Role of purinergic signaling pathways in V-ATPase recruitment to apical membrane of acidifying epididymal clear cells. Am. J. Physiol. Cell Physiol. 298(4): C817-C830.