ADP/ATP Ratio Assay Kit, BioAssay(TM)

Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP is much more pronounced in necrosis versus apoptosis.\n\nADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration.\nLuciferase ATP+D-luciferin+O2 oxyluciferin+AMP+PPi+CO2+light\nIn the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step.\n\nThis non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.\n\nKey Features:\nSafe. Non-radioactive assay.\nHomogeneous and convenient. "Mix-incubate-measure" type assay.\nNo wash and reagent transfer steps are involved.\nRobust and amenable to HTS: Z

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SPECIFICATIONS

Catalog Number

A0901-76A

Size

1Kit

References

1. Schafer ZT, et al. (2009). Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment. Nature. 461(7260):109-113.\n2. Bekeredjian R, et al. (2010). Conditional HIF-1alpha expression produces a reversible cardiomyopathy. PLoS One. 5(7): e11693.\n3. Chandak PG, et al. (2010). Efficient phagocytosis requires triacylglycerol hydrolysis by adipose triglyceride lipase. J Biol Chem. 285(26):20192-201.