ADP Colorimetric/Fluorometric BioAssay(TM) Kit

ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. \n\nADP BioAssay(TM) Kit provides an convenient colorimetric and fluorometric means to measure ADP level. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (Lmax = 570nm) or fluorometric method (Ex/Em 535/587nm). The assay is simple, sensitive, stable and high- throughput adaptable. The assay can detect as low as 1uM ADP in biological samples.\n\nKit Components:\n1. ADP Assay Buffer: 1x25ml\n2. ADP Probe (lyophilized): 1x1vial \n3. DMSO (anhydrous): 1x0.4ml \n4. ADP Converter: 1x1vial \n5. ADP Developer Mix: 1x1vial \n6. ADP Standard (1umol lyophilized): 1x1vial \n\nStorage and Stability:\nStore kit at -20 degrees C. Protect from light. Allow Assay Buffer to warm to room temperature \nbefore use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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