ADP Ribosylation Factor 1 (ARF1)

ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARFs family which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) is localizes in the cytoplasm while the active form (ARF1-GTP) is localized in the membrane (2-3). Active ARF1 also localized to the Golgi complex, regulates the assembly of coat proteins onto membranes and mediates traffic in the ER-Golgi. ATF1 activates phospholipase D and phosphatidyllino- sitol 4-phosphate 5-kinase and recruits COPI to membranes (4-5).\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:80.\nWestern Blot: 1:2000.\nImmunohistochemistry: 1:250-500.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

A0901-80A

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Bobak et al. Proc. Nat. Acad. Sci. 86: 6101-6105, 1989. 2. Serafini, T., Orci, L., Amherdt, M., Brunner, M., Kahn, R. A., and Rothman, J. E. (1991) Cell 67, 239

Additional Info

Recognizes human ARF1 at 21kD. Predicted to detect splice isoforms 3, 4, 5 based on sequence analysis.\nSpecies Crossreactivity: mouse and rat.\n