ADP Ribosylation Factor (ARF)

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21kD proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. \n\nApplications: \nSuitable for use in Immunofluorescence, Immunoprecipitation, Immunocytochemistry and Western Blot. Cannot be used to neutralize Arf in solution. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:500. Staining of Arf in rat pancreas results in staining of the cytoplasmic face of the trans-Golgi membranes. \nWestern Blot: 1:500. Detects a single ~21kD protein representing Arf in rat pancreas extract\nImmunoprecipitation: 1:50\nOptimal dilutions to be determiend by the researcher.\n\nCellular Localization: Golgi apparatus\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

A0902-10

Size

100ul

Applications

ICC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

J. Biol. Chem., 271(36): 21767-21774, 1996. General: Cell, 67: 239-253, 1991 .J. Cell Bio.,140(3): 511-523, 1998. J. Cell Biol., 138(3): 505-515, 1997. Proc. Natl. Acad. Sci. USA, 89; 6619-6623, 1992. Trends in Biochemical Sciences, 20: 147-150, 1995.

Additional Info

Recognizes human ADP-ribosylation factor1 (Arf 1), Arf 3, Arf 5 and Arf 6 and at about a ten-fold reduction with Arf 4. Species Crossreactivity: rat, mouse, canine and hamster.