ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1, GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein Ligase TRIM23, RING Finger Protein 46, RNF46)

Supplier:

United States Biological

Cat no: A3330-11A

ADP-Ribosylation Factor Domain Protein 1 (ARD1, ARFD1, ARF domain protein 1, GTP-binding Protein ARD-1, Tripartite Motif Protein 23, TRIM23, E3 Ubiquitin-protein Ligase TRIM23, RING Finger Protein 46, RNF46)

The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARFBP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunocytochemistry: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nDaudi cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A3330-11A

Size

50ug

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Gallagher SJ, et al (2006) Int. J. Biochem. Cell Biol. 38:1637-41. 2. Pomerantz J, et al (1998) Cell. 92:725-34. 3. Chen D, et al (2005) Cell. 121:1071-83. 4. Zhong Q, et al (2005) Cell. 121:1085-95.

Additional Info

Recognizes human ARF-BP1. Species Crossreactivity: mouse and rat.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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