Adrenergic Receptor, beta 3 (B3AR, BAR3, ADRB3R, Beta-3 adrenoreceptor)

The beta 3-adrenergic receptor (B3AR) is a member of the super-family of G protein-coupled receptor superfamily. They are characterized by seven putative transmembrane helices connected by hydrophilic loops. The mechanism by which the activated beta ARs transmit the signals across the plasma membrane involves the stimulation of Gs. This stimulation in turn activates adenyl cyclase. Activation of adenyl cyclase yields the second messenger cAMP. B3AR is primarily present in adipocytes The B3AR plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In humans, B3AR, a Trp to Arg replacement has recently been discovered at position 64. This change in the first coding exon has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome, and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (over 30%) and Japanese (20%). Chronic stimulation of the B3AR by highly selective B3AR agonist CL316,243, induces ectopic expression of UCP1 in adipose tissues and skeletal muscle. This expression may contribute to the potent anti-obesity effect of the beta 3-adrenergic agonist. Human B3AR is a 408aa membrane protein (rat/mouse, 400aa). The N-terminus is predicted to be extracellular; the C-terminus intracellular. \n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 \nELISA: 1:10,000-1:100,000. Coat with 50-100ng Control Peptide/well\nOptimal dilutions to be determined by researcher.\n\nControl Peptide: A0906-94\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nMouse Related Products:\nA0906-92: Adrenergic Receptor, beta 3, Mouse (B3AR) (Control Peptide)\nA0906-92A: Adrenergic Receptor, beta 3 (B3AR) Pab Rb xMo\nA0906-92B: Adrenergic Receptor, beta 3 (B3AR) Pab Rb xMo

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SPECIFICATIONS

Catalog Number

A0906-91

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Barak, L.S., et al., Biochem. 34: 15407 (1995). 2. Emorine, L.J., et al., Science 245: 1118 (1989). 3. Van Spronsen, A., et al., Eur. J. Biochem. 213: 1117 (1993). 4. Lelias, J.M., et al., FEBS Lett. 324: 127 (1993). 5. Grannenman, J.G., et al., Mol. Pharmacol. 42: 964-970 (1992).

Additional Info

Recognizes human Adrenergic Receptor beta 3, splice form 3. Species Sequence Homology: Canine (68%) and human B3AR splice form 2 (68%). No significant homology with the mouse or rat B3AR.