Adrenomedullin (ADML)

The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1 and CGRP2) and adrenomedullin (ADM). Adrenomedullin (ADM) is a 52aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADM is produced in peripheral tissues, adrenal medulla, lung and kidney. ADM has specific receptors on astrocytes and it is unregulated in ischaemia. The calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.\nAM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, AM is diuretic and natriuretic, and both AM and PAMP inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:50,000. Control peptide can be used to coat plate at 1ug/ml. See Cat. #A0909-40B.\nWestern Blot: 1:1000-1:5000 (ECL)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0909-40

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kitamura, K., et al., BBRC 194: 720 (1993). 2. Kitamura, H., et al., BBRC 192: 553 (1993). 3. Samson, W.K., et al., Front. Neuroendocrinol. 19: 100 (1998). 4. Champion, H.C., et al., Regul. Pept. 85: 1 (1999). 5. McLatchie, L.M., et al., Nature 393: 333-339 (1998). 6. Nagae, T., et al., BBRC 270: 89-93 (2000). 7. Husmann, K., et al., Mol. Cell Endocrinol. 162: 35-43 (2000).

Additional Info

Recognizes human ADML. No significant sequence homology was found with other calcitonin family of peptides.