Adrenomedullin (ADML)

The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADML). Calcitonin is 32aa peptide found in the parafollicular "C" cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulated the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption. CGRP is a 37aa peptide produced by tissue specific processing of the calcitonin gene. Calcitonin is the major product in the thyroid, whereas CGRP is the major product in neural tissues. CGRP is a potent cardiovascular agent. It has structural similarity with amylin. CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3aa. Amylin is a 37aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. Adrenomedullin (ADML) is a 52aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADML is produced in peripheral tissues, adrenal medulla, lung, and kidney. ADML has specific receptors on astrocytes and it is unregulated in ischaemia. The calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADML receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 0.5-1ug/ml; A0909-40B: Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0909-40A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kitamura, K., et al., BBRC 194: 720 (1993). 2. Kitamura, H., et al., BBRC 192: 553 (1993). 3. Samson, W.K., et al., Front. Neuroendocrinol. 19: 100 (1998). 4. Champion, H.C., et al., Regul. Pept. 85: 1 (1999). 5. McLatchie, L.M., et al., Nature 393: 333-339 (1998). 6. Nagae, T., et al., BBRC 270: 89-93 (2000). 7. Husmann, K., et al., Mol. Cell. Endocrinol.162: 35-43 (2000).

Additional Info

Recognizes human Adrenomedullin. No significant sequence homology was found with other calcitonin family of peptides.