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AES, CT (Amino Enhancer of Split, Amino-terminal Enhancer of Split, AES 1, AES-1, AES 2, AES-2, Esp1, Gp130-associated Protein GAM, Groucho Homolog, GRG, GRG Protein, GRG5, Protein ESP1, TLE5)

Cat no: A0921-80F

AES, CT (Amino Enhancer of Split, Amino-terminal Enhancer of Split, AES 1, AES-1, AES 2, AES-2, Esp1, Gp130-associated Protein GAM, Groucho Homolog, GRG, GRG Protein, GRG5, Protein ESP1, TLE5)

Applications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: human 293 cell lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0921-80F

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Miyasaka, H. et al. (1993) Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 216: 343352. 2. Jan, Y. et al. (2004) A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors. Cell. 116: 751762. 3. Tetsuka, T. et al. (2000) Inhibition of nuclear factorkappaBmediated transcription by association with the aminoterminal enhancer of split, a Grouchorelated protein lacking WD40 repeats*. J. Biol. Chem. 275: 43834390.

Additional Info

Recognizes human AES (C-TERMINAL). Species Crossreactivity: mouse and rat.

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