Afadin, phosphorylated (Ser1718) (ALL1-fused Gene from Chromosome 6 Protein, AF6, FLJ34371, MLLT4, Protein AF-6, RP3-431P23.3)

In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium (reviewed in 1). Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet (reviewed in 2). The actin filament-binding protein, Afadin, binds to nectin forming a connection to the actin cytoskeleton (3). AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these\njunctions are then involved in the formation and maintenance of TJs (4, 5). Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed\npredominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin as well the carboxyl-terminal F-actin binding region (6). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (7). Recent\nwork has also shown that Afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (8, 9). Phosphorylation at Ser1718 was discovered using an Akt substrate antibody.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0921-97

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. (2) Harris, T.J. and Tepass, U. (2010) Nat Rev Mol Cell Biol 11, 502-14. (3) Ikeda, W. et al. (1999) J Cell Biol 146, 1117-32. (4) Sato, T. et al. (2006) J Biol Chem 281, 5288-99. (5) Ooshio, T. et al. (2007) J Cell Sci 120, 2352-65. (6) Mandai, K. et al. (1997) J Cell Biol 139, 517-28. (7) Prasad, R. et al. (1993) Cancer Res 53, 5624-8. (8) Miyata, M. et al. (2009) J Cell Sci 122, 4319-29. (9) Miyata, M. et al. (2009) J Biol Chem 284, 24595-609.

Additional Info

Recognizes endogenous levels of l-afadin protein only when phosphorylated at serine 1718. Species Crossreactivity: canine, monkey, mouse, rat.

Alternative Names

Myeloid/lymphoid or Mixed-lineage Leukemia (Trithorax Homolog, Drosophila); Translocated to, 4