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AFX, phosphorylated (Ser193)

Cat no: A0928

AFX, phosphorylated (Ser193)

The Forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family, three members (FKHR, AFX and FKHRL1) have sequence similarity to the nematode homologue DAF- 16, which has been shown to mediate signaling via a pathway involving IGFR1, PI3K and Akt. There are three Akt phosphorylation sites in the FKHR proteins: Thr24, Ser256 and Ser319. Phosphorylation of FKHR family members at these sites by Akt promotes cell survival and regulates the cell cycle. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. \n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nMolecular Weight: 65kD\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0928

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

(1) Anderson, M.J. et al. (1998) Genomics 47, 187

Additional Info

Phospho-AFX (Ser193) Antibody detects endogenous levels of AFX only when phosphorylated at Ser193. Species Crossreactivity: Human, mouse, hampster.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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