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AFX, phosphorylated (Ser197) (Forkhead Box Protein O4, Fork Head Domain Transcription Factor AFX1, FOXO4, AFX1, MLLT7)

AFX, phosphorylated (Ser197) (Forkhead Box Protein O4, Fork Head Domain Transcription Factor AFX1, FOXO4, AFX1, MLLT7)

Cat no: A0928-02A

Supplier: United States Biological
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Several studies indicate that FKHR and AFX, mammalian homologues of the Caenorhabditis elegans forkhead transcription factor DAF-16, function in the insulin signaling pathway promoter contains one IRS, while in the IGFBP-1 promoter two copies of IRS are arranged as an inverted palindrome. Three tandem copies of IRSs are present in the G6Pase. AFX has been recently shown to serve as a substrate of PKB both in vitro and in vivo, and its transcriptional activity is inhibited by phosphorylation. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1:500-1:1,000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0928-02A
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes AFX, phosphorylated at (Ser197). Species Cross-Reactivity: human, mouse, rat.

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