AGAP2 (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 2, AGAP-2, Centaurin-gamma-1, Cnt-g1, GTP-binding and GTPase-activating Protein 2, GGAP2, Phosphatidylinositol-3-kinase Enhancer, PIKE, CENTG1, KIAA0167)

Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse brain cell lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0929-02B

Size

50ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Ye K, Hurt KJ, Wu FY, et al. Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N. Cell 2000; 103:919-30. 2. Jackson TR, Kearns BG, and Thiebert AB. Cytohesins and centaurins: mediators of PI3-kinase-regulated Arf signaling. Trends Biochem. Sci. 2000; 25:489-95. 3. Rong R, Ahn JY, Huang H, et al. PI3 kinase enhancer-Homer complex couples mGluRI to PI3 kinase, preventing neuronal apoptosis. Nat. Neurosci. 2003; 6:1153-61. 4. Ahn J-Y, Rong R, Kroll TG, et al. PIKE (Phosphotidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion. J. Biol. Chem. 2004; 279:16441-51. 5. Ahn J-Y, Hu Y, Kroll et al. PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt. Proc. Natl. Acad. Sci. USA 2004; 101:6993-8.

Additional Info

Recognizes human PIKE. Species Crossreactivity: mouse.