AGAP2, CT (Arf-GAP with GTPase, ANK Repeat and PH Domain-containing Protein 2, AGAP-2, Centaurin-gamma-1, Cnt-g1, GTP-binding and GTPase-activating Protein 2, GGAP2, Phosphatidylinositol-3-kinase Enhancer, PIKE, CENTG1, KIAA0167)

Phosphoinositide 3-kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C- terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Over-expression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0929-02C

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

References

1. Ye K. et al. Cell 10, 919 (2000). 2. Jackson TR. et al. Trends Biochem. Sci. 25, 489 (2000). 3. Rong R. et al. Nat. Neurosci. 6, 1153 (2003). 4. Ahn, JY. et al. J. Biol. Chem. 279, 16441 (2004). 5. Ahn, JY. Et al. Proc. Natl. Acad. Sci. USA 101, 6993 (2004).

Additional Info

Recognizes human PIKE. Species Crossreactivity: mouse.