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Aggrecan (Aggrecan Core Protein, ACAN, AGCAN, Aggrecan 1, Aggrecan1, AGC 1, AGC1, AGC1 Protein, Antigen Identified by Monoclonal A0122, Cartilage Specific Proteoglycan Core Protein, CSPCP, CSPGCP, Chondroitin Sulfate Proteoglycan 1, Chondroitin Sulfate Pr

Cat no: A1059-51B

Aggrecan (Aggrecan Core Protein, ACAN, AGCAN, Aggrecan 1, Aggrecan1, AGC 1, AGC1, AGC1 Protein, Antigen Identified by Monoclonal A0122, Cartilage Specific Proteoglycan Core Protein, CSPCP, CSPGCP, Chondroitin Sulfate Proteoglycan 1, Chondroitin Sulfate Pr

A1059-51B recognises human aggrecan, a proteoglycan and member of the aggrecan/versican proteoglycan family, which forms a major component of the extracellular matrix (ECM) of both cartilage and the central nervous system (CNS). Studies using a panel of core protein-directed antibodies against human aggrecan, revealed the distribution of different aggrecan isoforms within the CNS, and sub-divided the isoforms into clusters 1-5 accordingly. These studies also showed a difference in the relative abundance of these isoforms when comparing brain and cartilage tissues.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Paraffin and frozen sections\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNS1 myeloma cells with spleen cells from Balb/c mice.\n\nPositive Control: \nCerebral cortex\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1059-51B

Size

2ml

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Bov

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

Additional Info

Recognizes human articular cartilage aggrecan. Recognizes an epitope within the 0-glycan attachment site and has been identified as a cluster 2 isoform. Species Crossreactivity: bovine. Does not react with chicken, fish, rat.

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