Aggrecan FFGxx (Proteoglycan, PG, AGC 1, Aggrecan 1, Antigen identified by monoclonal A0122, Cartilage specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, CSPG1, CSPGCP, Large aggregating proteoglycan, MSK16, SEDK)

This fragment is rapidly released from the tissue when MMP catabolism of aggrecan occurs and has been identified in synovial fluid samples from patients with degenerative joint diseases.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1059-54M

Size

1ml

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Bov, GP

Form

Supplied as a liquid in 0.2M Tris HCll, pH 7.4, 01-10% Fetal Calf Serum, 0.002% sodium azide.

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG2b,l

References

This product has been used in:Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). Caterson B et al. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333-44 (2000).

Additional Info

Recognizes the N-terminal neoepitope sequence (FFGV....) generated at the ?MMP cleavage site? after MMP catabolism in the interglobular domain of aggrecan between amino acids ..PEN341 and 342FFG.. (Human sequence enumeration). Detects bands between approximately 60 and 150kD. Species Crossreactivity: Crossreacts with Human, Rat, bovine, Guinea pig, Horse and Pig.