Agrin

Supplier:

United States Biological

Cat no: A1059-60D

Agrin

Agrin is an extracellular matrix component which promotes the clustering of nicotinic acetylcholine receptors and other proteins during development at the neuromuscular junction (4). Agrin is a basal lamina protein important in the initial steps of postsynaptic differentiation at the neuromuscular junction. Cloning has revealed that rat agrin is a 220kD protein which contains several different structural motifs. The C-terminal half of agrin also contains 3 sites of alternative splicing referred to as X, Y and Z positions. While no difference in functional activity has been detected between splicing variants at site X (5), insertion of a 4 amino acid peptide at site Y modestly increases agrins nAChR clustering activity. The most dramatic differences in activity between isoforms are observed at splicing site Z. Insertion of an 8aa peptide at this position increases the clustering activity of soluble rat agrin about 10,000 fold (5). Expression of agrin isoforms containing the 8aa or 11aa inserts, or both, is restricted to the CNS (6). In contrast, agrin forms containing no insert at site Z show a widespread expression pattern including muscle. Agrin expression in brain and spinal cord is highest early in development coinciding with the peak of synaptogenesis, and the splicing pattern changes during development (6).\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry and Microarrays. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A1059-60D

Size

50ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Groffen, A.J., et al., J. Histochem. Cytochem. 46: 19

Additional Info

Recognizes rat Agrin at ~100kD. Species Crossreactivity: mouse.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

More from
United States Biological

All suplier products

United States Biological

FSD2, NT (FSD2, SPRYD1, Fibronectin type III and SPRY domain-containing protein 2, SPRY domain-containing protein 1) (FITC)

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

United States Biological

POU6F2, NT (POU6F2, RPF1, POU domain, class 6, transcription factor 2, Retina-derived POU domain factor 1) (FITC)

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

United States Biological

CEACAM18, NT (CEACAM18, Carcinoembryonic antigen-related cell adhesion molecule 18) (FITC)

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

United States Biological

LIPC, ID (LIPC, HTGL, Hepatic triacylglycerol lipase, Lipase member C) (FITC)

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

United States Biological

GNE, NT (GNE, GLCNE, Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-GlcNAc-2-epimerase/ManAc kinase, UDP-N-acetylglucosamine 2-epimerase, UDP-GlcNAc-2-epimerase, Uridine diphosphate-N-acetylglucosamine-2-epimerase, N-acet

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

United States Biological

TNFSF4, ID (TNFSF4, TXGP1, Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40 ligand, TAX transcriptionally-activated glycoprotein 1, CD252) (FITC)

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

United States Biological

F2RL2, NT (F2RL2, PAR3, Proteinase-activated receptor 3, Coagulation factor II receptor-like 2, Thrombin receptor-like 2) (FITC)

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

United States Biological

GBA3, CT (GBA3, CBG, CBGL1, Cytosolic beta-glucosidase, Cytosolic beta-glucosidase-like protein 1) (FITC)

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Advertisehere

MtoZ Horizontal banner

Latest promotion FROM:

Latest promotions

Explore all promotions

B-Bridge International, Inc.

DNA Cleanup in 7 Minutes, 10% OFF

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

BD (Becton, Dickinson and Company)

Analyze your cells with more colors at lower prices!

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

Europa Bioproducts Ltd

20% discount on Bovine Serum Albumin!

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

Cecil Instruments Limited

Cecil Spectrophotometer Enhancements for Free!

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

Cayman Chemical Company

Cayman Challenge

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

SDIX

Custom Polyclonal Antibodies

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Advanced Targeting Systems, Inc.

Secondary Conjugates - Save 20%

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Innovative Research, Inc

Premium US Origin Fetal Bovine Serum for only $95 per 500ml!

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Cell Sciences, Inc.

Recombinant Proteins

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Bibby Scientific

73 series spectrophotometer

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Everest Biotech Ltd

Custom antibody for $300

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...

Top suppliers

Explore all suppliers

United States Biological

230753 products

Carl Zeiss Microscopy

27 products

Promega Corporation

11 products

Panasonic Healthcare Company

5 products

Life Technologies

1 products

Nikon Instruments Europe

11 products

Olympus Europa Holding GmbH

3 products

Leica Microsystems, Inc.

10 products

GE Healthcare Life Sciences

2 products

Tecan Trading AG

19 products

Beckman Coulter, Inc.

1 products

AB SCIEX

3 products

BD (Becton, Dickinson and Company)

1 products

RANDOX TOXICOLOGY

5 products

Randox Food Diagnostics

6 products