Agrin, Recombinant, Human, 6-His-Tag

Agrin is a 400-600kD heparan sulfate proteoglycan component of the extracellular matrix. The N-terminal half of human Agrin, which mediates ECM interactions, contains a Laminin-binding NtA domain, nine Kazal-type protease inhibitor domains, two Laminin EGF-like domains, and one SEA domain. The C-terminal half contains four EGF-like repeats and three Laminin globular G domains. Rat Agrin lacks the NtA domain, and mouse and chick Agrin include the NtA domain only by the use of an alternate promoter. Additional isoforms are generated by alternate splicing at sites Y and Z in the C-terminal half of rat Agrin (known as A and B, respectively in chick). Agrin isoforms that contain an insert at site Z (Z+ forms) are known as neural Agrin and are selectively produced by motoneurons. Other isoforms are known as muscle Agrin and are additionally expressed in non-neuronal tissues, particularly in basement membranes of the lung and kidney (1-3). This recombinant protein consists of the C-terminal half of human Agrin corresponding to the Z- isoform of rat Agrin. It shares 60-78-80aa sequence identity with comparable regions of chick, mouse, and rat Agrin, respectively. The C-terminal half of Z-and Z+ Agrin binds to a-Dystroglycan and mediates adhesion between motoneurons and myotubes at the neuromuscular junction (NMJ) (4-6). In contrast, only Z+ Agrin is effective at inducing clustering of the postsynaptic Acetylcholine Receptor (AChR) and presynaptic motoneuron differentiation (7, 8). Agrin-induced AChR clustering requires a myotube receptor complex that contains a-Dystroglycan, MuSK, and LRP4 (4, 9-11). Agrin exhibits many functions in addition to NMJ development. It is enriched in senile Alzheimer

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SPECIFICATIONS

Catalog Number

A1059-60P

Size

50ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 300ug/ml.

Purity

~95% (SDS-PAGE)

References

1. Oike, Y. et al. (2009) Circ. J. 73:2192. 2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70. 3. Zeng, L. et al. (2003) J. Hum. Genet. 48:159. 4. Romeo, S. et al. (2009) J. Clin. Invest. 119:70. 5. Liu, D.J. and S.M. Leal (2010) PloS Genet. 6:e1001156. 6. Zhang, C.C. et al. (2006) Nat. Med. 12:240. 7. Zhang, C.C. et al. (2008) Blood 111:3415. 8. Khoury, M. et al. (2010) Stem Cells Dev. Dec 8 epub.