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AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)

AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)

Cat no: A1059-62M

Supplier: United States Biological
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Produced in goats immunized with purified, insect cell line Sf 21-derived, recombinant human Agouti-Related Protein (rhAgRP), also known as the protein product of Agouti-related transcript (ART). AgRP specific IgG was purified by human AgRP affinity chromatography and then biotinylated. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: Can be used as a detection antibody in a human AgRP ELISA in combination with the monoclonal capture antibody (Catalog# A1059-62G). Using plates coated with 100ul/well of the capture antibody, in combination with 100ul/well of the detection antibody at 50ng/ml, an ELISA for sample volumes of 100ul can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard stAgRPing with 4ng/ml. Western Blot: 0.1-0.2ug/ml. The detection limit for rhAgRP is approximately 1ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1059-62M
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human AgRP. ~40% cross-reactivity with rmAgRP.

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