AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-related transcript) (Biotin)

Produced in goats immunized with purified, insect cell line Sf 21-derived, recombinant human Agouti-Related Protein (rhAgRP), also known as the protein product of Agouti-related transcript (ART). AgRP specific IgG was purified by human AgRP affinity chromatography and then biotinylated. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: Can be used as a detection antibody in a human AgRP ELISA in combination with the monoclonal capture antibody (Catalog# A1059-62G). Using plates coated with 100ul/well of the capture antibody, in combination with 100ul/well of the detection antibody at 50ng/ml, an ELISA for sample volumes of 100ul can be obtained. To arrive at the optimal dose range for \nthis ELISA, set up a two-fold dilution series of the protein standard stAgRPing with 4ng/ml. \nWestern Blot: 0.1-0.2ug/ml. The detection limit for rhAgRP is approximately 1ng/lane under non-reducing and reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1059-62M

Size

50ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 50ug BSA. Reconstitute with sterile Tris-buffered saline pH 7.3 (20mM Tris base, 150mM sodium chloride) containing 0.1% BSA. If 1ml of buffer is used, the antibody concentration will be 50ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n

Isotype

IgG

Additional Info

Recognizes human AgRP. ~40% cross-reactivity with rmAgRP.