Ah Receptor (Aryl Hydrocarbon Receptor, AHR)

The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000. Detects an ~95kD protein representing AhR from Hepa 1 cytosol.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1059-72Z

Size

100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.05% sodium azide

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Ray SS & Swanson HI Dioxin-induced immortalization of normal human keratinocytes and silencing of p53 and p16INK4a. J Biol Chem 279:27187-93 (2004). 2. Delescluse C et al. Is CYP1A1 induction always related to AHR signaling pathway? Toxicology 153:73-82 (2000). 3. Perdew GH et al. Production and characterization of monoclonal antibodies directed against the Ah receptor. Hybridoma 14:279-83 (1995). 4. Chen HS & Perdew GH Subunit composition of the heteromeric cytosolic aryl hydrocarbon receptor complex. J Biol Chem 269:27554-8 (1994).

Additional Info

Recognizes mouse Aryl hydrocarbon receptor. Species Crossreactivity: human and rat

Alternative Names

Dioxin Receptor