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AHA1, Recombinant, Human (Activator of Hsp90 ATPase 1)

Cat no: A1059-70A

AHA1, Recombinant, Human (Activator of Hsp90 ATPase 1)

The co-chaperone Aha1 (Activator of HSP90 ATPase-1) binds the middle domain of Hsp90 (aa272-627) and is proposed to function as an ATPase-activating protein that competes with p23 and other co-chaperones for Hsp90 binding. \n\nSource: \nRecombinant human, AHA1, full length, with C-terminal HIS tag, expressed in E. coli.\n\nMolecular Weight: 38kD\n\nSpecific Activity: \nThe addition of AHA1 increased ATPase activity of hsp90-alpha from 0.18 to 0.94pmol/min/ug.\n\nAssay conditions: \n50ul reaction mix (100mM TrisHCl pH7.4, 20mM KCl, 6mM MgCl2, 200uM ATP, and 5ug Hsp90a) was incubated with Aha1 (0-10g) for 3 hrs at 37 degrees C. To stop the reaction, 100ul of the malachite green reagent was added to each well. Absorbance at 620nm was measured. \n\nApplications: \nSuitable for use as ATPase activity assay for Hsp90-alpha. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

A1059-70A

Size

200ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

Purity

(same/more than)90%

References

1. Wang, X. et al., Cell 127 (4), 803815 (2006). 2. Lotz, G.P. et al., J. Biol. Chem. 278 (19), 1722817235 (2003).

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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