AHCY (Adenosylhomocysteinease) Recombinant, Human

Human S-Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD\ncofactor for each subunit (1 2). It is the only known enzyme to catalyze the breakdown of\nSadenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under\nphysiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCYпїЅs\nactivity in mammals is directly related to homocysteine level, an independent risk factor for\nvascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adonosylLmethionine\nmethyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S-adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4 5).\n\nSource: \nRecombinant corresponding to Ser2-Tyr432 from human Adenosylhomocysteinease/AHCY, N-terminal Met, 6-His tag, expressed in E. coli.\n\nMolecular Weight: \n~49kD\n\nBiological Activity:\nMeasured by the hydrolysis of S-adenosyl-L-homocysteine. The specific activity is >350 pmoles/min/ug, as measured under the described conditions.\n\nEndotoxin Level:\n<1EU/ug (LAL method)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1059-73G

Size

10ug

Form

Supplied as a liquid in Tris, sodium chloride, Glycerol.

Purity

~95% (SDS-PAGE)

References

1. Turner, M. A. et al. (2000) Cell Biochem. Biophys. 33:101. 2. Takata, Y. et al. (2002) J. Biol. Chem. 277:22670. 3. Gellekink, H. et al. (2004) Eur. J. Hum. Genet. 12:942. 4. Baric, I. et al. (2004) Proc. Natl. Acad. Sci. USA. 101:4234. 5. Fumic, K. et al. (2007) Eur. J.Hum. Genet. 15:347.