AIB 1 (Amplified in Breast Cancer-1)

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Amplified in breast cancer-1 (AIB-1), is a member of a growing family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC-1) and glucocorticoid receptor interacting protein-1 (GRIP1). AIB1:RAC3 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, progesterone, and peroxisome proliferator activated receptors. AIB1:RAC3 also interacts with the transcriptional integrators CREB binding protein (CBP) and p300.\n\nApplication(s): \nWestern Blot\n\nRecommended Dilution:\nWestern Blot: Predicted molecular weight: 155kD. Can be blocked with AIB 1 peptide.\nOptimal dilution to be determined by the researcher.\n\nPositive Control:\nT47D cell lysate \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0857-05

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Xen/Amph

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Anzick SL et al. AIB1, a steroid receptor coactivator amplified in breast and ovarian cancer. Science 277:965-8 (1997). 2. Li H et al. RAC3, a steroid/nuclear receptor-associated coactivator that is related to SRC-1 and TIF2. Proc Natl Acad Sci U S A 94:8479-84 (1997). 3. Takeshita A et al. TRAM-1, A novel 160kD thyroid hormone receptor activator molecule, exhibits distinct properties from steroid receptor coactivator-1. J Biol Chem 272:27629-34 (1997).

Additional Info

Detects human amplified in breast cancer 1 (AIB1). Species Crossreactivity: Expected to crossreact with Xenopus laevis (92% identity with immunogen) due to sequence homology. \n\n\n