Home  >  Products  >  AICDA (Activation-induced Cytidine Deaminase, Cytidine Aminohydrolase, AID)
AICDA (Activation-induced Cytidine Deaminase, Cytidine Aminohydrolase, AID)

AICDA (Activation-induced Cytidine Deaminase, Cytidine Aminohydrolase, AID)

Cat no: A0855-75S

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation. The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function. AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 10ug/ml Western Blot: 1-2ug/ml Immunocytochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from Ramos cells Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A0855-75S
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunocytochemistry, Immunofluorescence, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6. 2. Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 2000; 102:553-63. 3. Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80. 4. Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8. 5. Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.
Additional info: Recognizes human AICDA. Species Crossreactivity: mouse.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave