Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number:
A0855-75U
Reactivities:
Human, Mouse, Rat
Hosts:
Rabbit
Applications:
ELISA, Immunohistochemistry, Western Blot
Size:
50ug
Form:
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
P type:
Pab
Isotype:
IgG
Purity:
Purified by immunoaffinity chromatography.
References:
1. Muramatsu, M. et al. J. Biol. Chem. 274,18470 (1999). 2. Muramatsu, M. et al. Cell 102, 553 (2000). 3. Greeve, J, et al. Blood 101, 3574 (2003). 4. Cascalho, M. J. Immunol. 172, 6513 (2004). 5. Sheehy, AM. et al. Nature 418, 646 (2002).
Additional info:
Recognizes human AID. Species Crossreactivity: rat and mouse.
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