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AIF (Apoptosis-inducing Factor 1 Mitochondrial, Apoptosis-inducing Factor Mitochondrion-associated 1, AIFM1, COXPD6, MGC111425, Programmed Cell Death Protein 8, PDCD8)

Cat no: A1059-78T

AIF (Apoptosis-inducing Factor 1 Mitochondrial, Apoptosis-inducing Factor Mitochondrion-associated 1, AIFM1, COXPD6, MGC111425, Programmed Cell Death Protein 8, PDCD8)

Apoptosis-inducing factor (AIF) is a phylogenetically ancient mitochondrial intermembrane flavoprotein. AIF induces caspase-independent peripheral chromatin condensation and large-scale DNA fragmentation when added to purified nuclei. Upon apoptosis induction AIF translocates to the cytosol and the nucleus.\n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 0.25-1microg/ml\nWestern Blot: 0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman, mouse, rat heart lysate, or K562 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1059-78T

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Marzo I. et al. FEBS Lett 427(2):198-202 (1998). 2. Susin S.A., et al. Nature. Feb 4;397(6718):441-6 (1999). 3. Cande C., et al. Biochimie 84(2-3):215-222 (2002).

Additional Info

Recognizes human AIF. Species Crossreactivity: mouse and rat.

Alternative Names

EC=1.-.-.-

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