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AIFM1 (Programmed Cell Death Protein 8, Apoptosis-inducing Factor 1, Mitochondrial, AIF, PDCD8)

AIFM1 (Programmed Cell Death Protein 8, Apoptosis-inducing Factor 1, Mitochondrial, AIF, PDCD8)

Cat no: A1372-32D

Supplier: United States Biological
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Apoptosis-inducing factor (AIF) is a phylogenetically ancient mitochondrial intermembrane flavoprotein. AIF induces caspase-independent peripheral chromatin condensation and large-scale DNA fragmentation when added to purified nuclei. Upon apoptosis induction AIF translocates to the cytosol and the nucleus. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml Immunohistochemistry (paraffin): 0.25-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human, mouse, rat heart lysate, or K562 cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1372-32D
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein G affinity chromatography.
References: 1. Marzo I. et al. FEBS Lett 427(2):198-202 (1998). 2. Susin S.A., et al. Nature. Feb 4;397(6718):441-6 (1999). 3. Cande C., et al. Biochimie 84(2-3):215-222 (2002).
Additional info: Recognizes human AIF. Species Crossreactivity: mouse and rat.

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