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AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein, APECED Protein, APECED)

AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein, APECED Protein, APECED)

Cat no: A1059-88N

Supplier: United States Biological
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AIRE (Autoimmune Regulator; also APECED) is a 58-60kD member of the PHD Zn-finger family of transcription factors. It appears to have restricted expression, being identified in thymic medullary epithelium, dendritic cells and neutrophils. AIRE regulates the expression of self-antigens in thymus. In particular, genes coding for these antigens are generally inactive, and show minimal H3K4me3 and AcH3 reactivity in their promoters. Human AIRE is 545aa in length. It contains multiple domains, including an HSR domain that mediates homodimerization (aa1-105), an NLS (aa131-133), a SAND domain (aa181-280), and two Zn-finger PHD domains (aa296-343 and 434-475) that bind to nonmethylated histone H3. There are isoforms that show a Trp substitution for aa437-467, a 95aa substitution for aa1-292, and a 75aa substitution for aa377-545. Over aa476-545, human AIRE shares 71% aa identity with mouse AIRE. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.3-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human spleen lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1059-88N
Reactivities: Human
Hosts: Goat
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Niki S, Oshikawa K, Mouri Y, Hirota F, Matsushima A, Yano M, Han H, Bando Y, Izumi K, Matsumoto M, Nakayama KI, Kuroda N, Matsumoto M. Alteration of intra-pancreatic target-organ specificity by abrogation of Aire in NOD mice. J Clin Invest. 2006 May;116(5):1292-301. Epub 2006 Apr 20. PMID: 16628255.
Additional info: Recognizes human AIRE.

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