AIRE (Autoimmune regulator; also APECED) is a 58-60kD transcriptional regulator that exhibits tight regulation of its expression. It is widely expressed in lymphoid organs, but is most pronounced in thymic medullary epithelium, where it induces the expression of organ-specific antigens that are used to sequester and delete autoreactive lymphocytes. Mouse AIRE is 552aa in length. It contains multiple domains, including an HSR domain with two LXXLL motifs (aa1-106), an NLS (aa114-134), one SAND domain (aa182-282) and two PHD zinc-finger regions (aa298-345; 434-475). AIRE will homodimerize and tetramerize. There are multiple potential isoforms that involve various combinations of deletions of aa265-268, 368-426 and Lys296, plus a 42aa substitution for aa368-552. Over aa476-552, mouse AIRE shares 91% and 65% aa identity with rat and human AIRE, respectively.
Applications:
Suitable for use in Western Blot Other applications not tested.
Recommended Dilution:
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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