Ajuba (an Urdu word meaning "curiosity") is a 55-60kD member of the Zyxin/Ajuba family of proteins. Ajuba is found in keratinocytes, astrocytes and neurons, and exhibits multiple functions. It shuttles between the nucleus and cytosol, and participates in alpha- catenin:F-actin binding, beta-catenin binding and phosphorylation, p62 aPKC scaffold protein binding and NF-kB activation, and Aurora-A binding and phosphorylation. Human Ajuba is 538aa in length. It contains a Pro-rich preLIM region that binds actin and Grb2 (aa1-335), an NES (aa280 -287), and three LIM domains that bind multiple proteins (aa336-397, 401-461 and 462-530). Ajuba is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:20,000-1:80,000
Western Blot: 1:500-1:2,500
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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