Ajuba (JUB, Ajuba Homolog (Xenopus laevis), MGC15563, Protein Ajuba)

Supplier:

United States Biological

Cat no: A1099-01D

Ajuba (JUB, Ajuba Homolog (Xenopus laevis), MGC15563, Protein Ajuba)

Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:100\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1099-01D

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, NHP

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

Additional Info

Recognizes endogenous levels of total Ajuba protein. Species crossreactivity: Mouse, monkey and bovine Species Sequence Homology: Rat.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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