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Ajuba (JUB, Ajuba Homolog (Xenopus laevis), MGC15563, Protein Ajuba)

Ajuba (JUB, Ajuba Homolog (Xenopus laevis), MGC15563, Protein Ajuba)

Cat no: A1099-01D

Supplier: United States Biological
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Ajuba belongs to the Zyxin/Ajuba family, members of which include LIMD1, LPP, Trip6 and Zyxin. Three LIM domains at the carboxy-terminus characterize this family of proteins. Zyxin/Ajuba family members associate with the actin cytoskeleton, are components of both cell-cell junction adhesive complex and integrin-mediated adhesive complex, and shuttle in and out of the nucleus (1). Ajuba is involved in Rac activation during cell migration and activates Aurora A in order to commit to mitosis (2-3). Ajuba and its family members also have an amino-terminal proline-rich pre-LIM region, and Ajuba augments ERK activation in a Ras-dependent manner (4). Applications: Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100 Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1099-01D
Reactivities: Human, Mouse, Rat, Bovine, Non-Human Primate
Hosts: Rabbit
Applications: Flow Cytometry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A and peptide affinity chromatography.
Additional info: Recognizes endogenous levels of total Ajuba protein. Species crossreactivity: Mouse, monkey and bovine Species Sequence Homology: Rat.

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