Home  >  Products  >  AKAP3 (A Kinase (PRKA) Anchor Protein 3, AKAP110, Fibrousheathin 1, FSP95, PRKA3, SOB1, Sperm Oocyte Binding Protein 1)
AKAP3 (A Kinase (PRKA) Anchor Protein 3, AKAP110, Fibrousheathin 1, FSP95, PRKA3, SOB1, Sperm Oocyte Binding Protein 1)

AKAP3 (A Kinase (PRKA) Anchor Protein 3, AKAP110, Fibrousheathin 1, FSP95, PRKA3, SOB1, Sperm Oocyte Binding Protein 1)

Cat no: A1102-80A

Supplier: United States Biological
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A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm. Cellular Localization: Localizes to the ribs of the fibrous sheath in the principal piece of the sperm tail. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000. Western Blot: 1-3ug/ml. Predicted molecular weight: 95kD. detects a band of ~ 90kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1102-80A
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Lefevre A et al. Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition. Biochem Biophys Res Commun 259:60-6 (1999).
Additional info: Species Crossreactivity: Tested: Human.

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