Akt Kinase Activity, Mammalian, BioAssay(TM) Kit

Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3a as substrate. Phosphorylation of the GSK-3a can be analyzed by Western blot analysis using the phospho-GSK-3a specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.\n\nSample type:\nCell and Tissue lysates\n\nIntended Use:\nThe Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3a as substrate.\n\nSpecificity:\nMammalian\n\nKit Components:\nKinase extraction buffer, 1x80ml\nAkt specific antibody, 1x80ul\nProtein a sepharose, 1x2ml\nGSK-3a protein/ATP mixture, 1x80ul\nKinase assay buffer, 1x25ml\nPhospho-GSK-3a specific antibody, 1x50ul\n\nStorage and Stability:\nStore at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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