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Akt, Phosphorylated (Ser473) (PKB, Protein Kinase B, RAC-alpha)

Akt, Phosphorylated (Ser473) (PKB, Protein Kinase B, RAC-alpha)

Cat no: A1124-02C

Supplier: United States Biological
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Akt, also known as Protein Kinase B (PKB) or RAC-alpha, is a 60 kD serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating many proteins including GSK-3Beta, caspase 9, BAD and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins such as FAK, when FAK is phosphorylated at tyrosine 397, thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at serine 473 is required for full activation of Akt. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:250 Optimal dilutions to be determined by the researcher. Positive Control: Mouse NIH3T3 cells, 3T3-L1 adipocytes Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1124-02C
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Kumar, P. et al. J. Biol. Chem. 279(41), 43352 (2004). 2. Kovalenko, D. et al. J. Biol. Chem. 278(16), 4087 (2003). 3. Beffert, U. et al. J. Biol. Chem. 277(51), 49958 (2002). 4. Morel, JC. et al. J. Biol. Chem. 277(38), 34679 (2002).
Additional info: Recognizes human Akt when phosphorylated at Ser473. Species Crossreactivity: mouse

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