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Akt, Phosphorylated (Ser473) (PKB, Protein Kinase B, RAC-alpha)

Akt, Phosphorylated (Ser473) (PKB, Protein Kinase B, RAC-alpha)

Cat no: A1124-07M

Supplier: United States Biological
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The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:15,000-1:60,000 Western Blot: 1:200-1:500 Immunohistochemistry: 1:100-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1124-07M
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes phosphorylated human AKT. Phospho specific for pS473. Minimal reactivity occurs against non-phosphorylated AKT. Species sequence homology: mouse, rat, chicken.

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