Akt, phosphorylated (Ser473) (Rac PKa, PKBa)

Akt is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser473 and Thr308. Akt is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). Akt is a key regulator of many signal transduction pathways. Akt exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of Akt is noted in many types of cancer. Akt mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits Akt to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, Akt dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. Akt has two main roles: inhibition of apoptosis and promotion of proliferation.\n \nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:15,000-1:60,000\nWestern Blot: 1:200-1:1000, Detects a band at ~55.7kD\nImmunohistochemistry (Formalin-fixed, Paraffin-embedded sections): 1:100-1:500. No pretreatment required.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1124-02B

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Lawlor, M.A. & Alessi, D.R., J. Cell Science 114: 2903-2910 (2001). 2. Alessi, D.R., Biochem. Soc. Trans. 29: 1-14 (2001).01).

Additional Info

Recognizes phosphorylated human Akt. Minimal reactivity occurs against non-phosphorylated Akt. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum. Species Crossreactivity: mouse and rat.