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Akt, Phosphorylated (Thr308) (PKB, Protein Kinase B, RAC-alpha)

Akt, Phosphorylated (Thr308) (PKB, Protein Kinase B, RAC-alpha)

Cat no: A1124-07G

Supplier: United States Biological
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Akt, also known as Protein Kinase B (PKB) or RAC-alpha, is a 60 kD serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3Beta, caspase 9, BAD, and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins like FAK (when FAK is phosphorylated at tyrosine 397), thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at threonine 308 is necessary for phosphorylation of serine 473, which is required for full activation of Akt. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 dilution Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cells, 3T3-L1 adipocytes. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1124-07G
Reactivities: Human, Mouse, Rat, Bovine, Chicken/Bird
Hosts: Rabbit
Applications: Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Persad, S. et al. J. Biol. Chem. 276(29), 27462 (2001). 2. Datta, SR. et al. Cell 91(2), 231 (1997). 3. Beffert, U. et al. J. Biol. Chem. 277(51), 49958 (2002). 4. Franke, TF. et al. Cell 88(4), 435 (1997).
Additional info: Recognizes human Akt1 when phosphorylated at Thr308. Species Crossreactivity: mouse, rat, chicken and cow.

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