Akt, phosphorylated (Thr308) (Rac PKa, PKBa)

Phosphorylation site specific antibody selective for the phosphorylated form of the AKT protein containing a phosphate on threonine 308. The antibody has been shown to recognize AKT in NIH-3T3 cells stimulated with PDGF. AKT is a key effector of the PI3K pathway. Several studies have shown that a signalling pathway from PI3K to AKT may mediate some cellular responses of PI3K, including protection from apoptosis in PC12 cells. AKT accomplishes its cell survival-promoting function by inhibiting apoptosis by means of its ability to phosphorylate and inactivate BAD and caspase 9. The function of AKT is regulated by phosphorylation on two sites conserved within the AGC kinase family: the activation loop (threonine 308) in the kinase core and hydrophobic phosphorylation site on the carboxyl terminus (serine 473). Threonine 308 is phosphorylated by the phosphoinositide-dependent kinase 1 (PDK 1).\n\nCellular Localization: Cytoplasmic and Nuclear\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1124-92C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Dulbecco's PBS [pH7.3] with 50% glycerol and 1mg/ml BSA containing 0.05% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Franke TF et al. PI3K: downstream AKTion blocks apoptosis. Cell 88:435-7 (1997). 2. Carpenter CL & Cantley LC Phosphoinositide kinases. Curr Opin Cell Biol 8:153-8 (1996). 3. Burgering BM & Coffer PJ Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376:599-602 (1995). 4. Franke TF et al. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81:727-36 (1995). 5. Beffert, U., et al. (2002). Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. JBC. 277(51): 49958-49964. 6. Hsu, J., et al. (2001). The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9): 2853-2855.7. Persad, S., et al. (2001). Regulation of protein kianse B/Akt-theronine-308 phosphorylation by integrin kinked kinase (ilk): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. JBC. 276(29): 27462-27469. 8. Nelson, J.M., & Fry, D.W. (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. JBC. 276(18): 14842-14847. 9. Genot, E.M., et al. (2000) The T-Cell receptor regulates Akt (Protein Kinase B)via a pathway involving Rac1 and phosphotidylinositide 3-kinase. Mol. Cell. Biol. 20(15): 5469-5478. 10. Datta, S.R., et al. (1997) Akt phosphoylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell. 91(2): 231-241. 11. del Peso, L., et al. (1997) Interleukin-2-induced phosphoylation of BAD through the protein kinase Akt. Science. 278(5338): 687-689. 12. Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kianse Akt. Science. 275(5300): 661-665.

Additional Info

Recognizes human Akt when phosphorylated at Thr308. Species Crossreactivity: Crossreacts with Human and Mouse.

Alternative Names

CAKT, Murine thymoma viral (vakt) oncogene homolog 1, PKB Akt, PKB alpha, PRKBA, RAC alpha serine/threonine protein kinase, RAC Serine/Threonine Protein Kinase, V AKT Murine Thymoma Viral Oncogene Homolog 1, VAKT Murine Thymona Viral Oncogene 1, RAC-PK-alpha, Protein kinase B