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Akt, Phosphorylated (Thr668308) (Rac PKa, PKBa)

Akt, Phosphorylated (Thr668308) (Rac PKa, PKBa)

Cat no: A1124-01Q

Supplier: United States Biological
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AKT, also known as Protein Kinase B (PKB) or RAC-a, is a 60kD serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3b, caspase 9, BAD, and the Forkhead Transcription Factor. AKT is activated by PI3K, which in turn can interact with proteins like FAK thereby linking activation to the cytoskeleton. AKT/PKB is constitutively active in many types of human cancer, and contributes to tumor progression by promoting angiogenesis and cell invasiveness. AKT is fully activated by phosphorylation of threonine 308 by PDK1 and serine 473 by PDK2. Phosphorylation at threonine 308 is necessary for phosphorylation of serine 473, which is required for full activation of AKT). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cells, 3T3-L1 adipocytes Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A1124-01Q
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Persad, S. et al. J Biol Chem 276, 27462 (2001). 2. Datta, S. et al. Cell 91, 231 (1997). 3. Beffert, U. et al. J Biol Chem 277, 49958 (2002). 4. Franke, T. et al. Cell 88, 435 (1997).
Additional info: Recognizes human Akt, when phosphorylated at threonine 308. Species Crossreactivity: mouse.

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