Akt (Rac PKa, PKBa)

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).\n\nCellular Localization: Cytoplasmic and Nuclear\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A1124-01D

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Species Crossreactivity: Crossreacts with Human.

Alternative Names

CAKT, Murine thymoma viral (vakt) oncogene homolog 1, PKB Akt, PKB alpha, PRKBA, RAC alpha serine/threonine protein kinase, RAC Serine/Threonine Protein Kinase, V AKT Murine Thymoma Viral Oncogene Homolog 1, VAKT Murine Thymona Viral Oncogene 1, RAC-PK-alpha, Protein kinase B