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Akt (Rac PKa, PKBa)

Akt (Rac PKa, PKBa)

Cat no: A1124-01F

Supplier: United States Biological
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AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3- kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: 1inhibition of apoptosis; 2 promotion of proliferation. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000 Western Blot: 1:500-1:3,000 Immunohistochemistry: 20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A1124-01F
Hosts: Mouse
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
References: 1.Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903- 2910. 2.Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. Jones,P.F., Jakubowicz,T., Pitossi,F.J., Maurer,F. and Hemmings,B.A. (1991) Molecular cloning and identification of aserine/threonine protein kinase of the second-messenger subfamily. Proc. Natl. Acad. Sci. U.S.A. 88 (10), 4171-4175. 3.Staal,S.P. (1987) Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma. Proc. Natl. Acad. Sci. U.S.A. 84 (14), 5034-5037.
Additional info: Recognizes human. Species Sequence Homology: rat and chimpanzee.

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