AKT (Rac PKa, PKBa)

The protein kinase B (PKB, also called AKT) family of protein kinases plays a key role in insulin signaling, cellular survival, and transformation. AKT1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 0.5-2ug/ml\nImmunoprecipitation: 3-5ug/extract\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1124-01P

Size

50ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Maira, S. et al. Science 294, 374 (2002). 2. Hemmings, B. et al. Science 275, 628 (2001) 3. Stephens, L. et al. Science 279, 710 (1998). 4. Alessi, D. et al. Curr Biol 7, 261 (1997). 5. Bellacosa, A. et al. Science 254, 274 (1991). 6. Jones, P. et al. Proc Natl Acad Sci USA 88, 4171 (1991).

Additional Info

Recognizes human AKT1. Species Crossreactivity: mouse and rat.